Relationships between Smoking, Age, Body Mass Index, and Abstinence with DNA Fragmentation Index in Male Infertile Patients

Atifa Nadira Ediningtyas, Abdurahman Laqif, Eriana Melinawati, Supriyadi Hari Respati


Background: Infertility increases annually, by 0.370% in women and 0.291% in men. One of the causes of male infertility is idiopathic in the form of cigarettes, alcohol, drugs, obesity, psychological stress, old age, dietary factors, and exposure to toxins in the environment or work. These factors can cause oxidative stress that can lead to sperm DNA damage or sperm DNA fragmentation as measured through the DNA Fragmentation Index (DFI). So this study to analyzed about the relationship between smoking, age, Body Mass Index, and abstinence period with DNA Fragmentation Index in infertile patient.

Subjects and Method: This type of research is observational analytics with a cross-sectional approach. The study was conducted at the Sekar Clinic of Dr. Moewardi Hospital Surakarta. A total of 35 subjects of male infertility patients performed DNA examinations of sperm fragmentation. Inde¬pendent variables: smoking, age, body mass index (BMI), and abstinence. Dependent variable: DFI. Data is collected using medical records and analyzed using SPSS applications through uni-variate, bivariate, and multivariate analysis (logistic regression).

Results: Smoking (OR= 0.68; 95% CI= 0.52 to 2.23; p= 0.636), age (OR= 17.33; 95% CI=31.06 to 35.05; p= 0.003), BMI (OR= 1.47; 95% CI= 24.20 to 26.52; p = 0.58), abstinence (OR= 5.67, 95% CI =3.76 to 4.69; p=0.02). Results of multivariate analysis with logistic regression at age (OR= 13.62; 95% CI =1.42 to 130.60; p=0.024), and abstinence (OR= 3.94; 95% CI= 0.84 to 21.58; p= 0.114).

Conclusion: There is a relationship between age and abstinence to DFI. There is no relationship between smoking and BMI against DFI.

Keywords: smoking, age, body mass index, abstinence, DNA fragmentation inde

Correspondence: Atifa Nadira Ediningtyas. Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Jawa Tengah, Indonesia. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java. Email: Mobile: 08112630104.

Journal of Maternal and Child Health (2022), 07(04): 398-406


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